The Bio Trace Elements Supplement including Sr, Li, Rb, Mn, Ba, V, Al, Mo, I, Ti, Fe, Co, Cu, Vitammin B1 and B12 etc. other trace elements which can supply the trace elements consumed by marine animals. Direction for Use： Premix the Bio Trace Elements Supplement totally into the water to be added. Calculate the Volume of aquarium water, and add the Bio Trace Elements Supplement according to the volume. A level of measuring spoon can measure Bio Trace Elements Supplement appro.10 g/0.35OZ wich can mix in 380 L/100 U.S.gallon water.Adding once time per week. Notes: Please don’t directly add the Bio Trace Elements to your tank. To aviod the stress reaction of marine animals, recommend to premix Bio Trace Elements before mixing.